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Exploring Multi Potential Uses of Marine Bacteria; An Integrated Approach for PHB Production, PAHs and Polyethylene Biodegradation

Exploring Multi Potential Uses of Marine Bacteria; An Integrated Approach for PHB Production, PAHs and Polyethylene Biodegradation

K Mohanrasu, N Premnath, G Siva Prakash, Muniyasamy Sudhakar, T Boobalan, A Arun

J Photochem Photobiol B. 2018 Aug;185:55-65.

PMID: 29864727

Abstract:

There are copious of bacteria exist in marine environment and it is very important to screen the potential microbes that has the ability to produce biopolymer polyhydroxybutyrate (PHB) as well as polycyclic aromatic hydrocarbons (PAHs) degradation and conventional plastic high density polyethylene (HDPE) biodegradation. Numerous studies have been investigated individually on either one of characteristic feature like PHB production, PAHs and high density polyethylene (HDPE) degradation, but not all together. Hence, in this study, we tried to screen potential marine microbes that have the ability to perform all three features. We have isolated 203 phenotyphicaly different colonies from 19 different sites (marine soil sediments, marine water and oil spilled marine water) which cover the north east to down south seashore regions of Tamilnadu, India. Of the 203 microbial isolates, the best PHB producing (Micrococcus luteus), PAHs degradation (Klebsiella pneumonia) and HDPE degradation (Brevibacillus borstelensis) microorganisms were identified through 16S rRNA sequencing. Analytical studies confirmed PHB production by fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance (1H &13C NMR); PAHs degradation by high performance liquid chromatography (HPLC), confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM); HDPE degradation by CLSM, FT-IR and SEM which cover the spectroscopy studies on biological systems.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AS26686	PAHs by HPLC			Price

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