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Expression and Characterization of Recombinant Human VEGF165 in the Middle Silk Gland of Transgenic Silkworms

Expression and Characterization of Recombinant Human VEGF165 in the Middle Silk Gland of Transgenic Silkworms

Tianyang Zhang, Rongpeng Liu, Qin Luo, Dawei Qu, Tao Chen, Ou Yao, Hanfu Xu

Transgenic Res. 2019 Dec;28(5-6):601-609.

PMID: 31541344

Abstract:

Recombinant human vascular endothelial growth factor (rhVEGF) has important applications in therapeutic angiogenesis and inhibition of VEGF-mediated pathological angiogenesis. Previous studies have shown that rhVEGF can be produced in several expression systems, including Escherichia coli, yeasts, insect cells and mammalian cells. However, little is known regarding the effective production of this protein in organs of live organisms. Here, we report for the first time the expression and characterization of rhVEGF165 in the middle silk gland (MSG) of the transgenic silkworm line S1-V165. Our results confirmed that (1) rhVEGF165 was highly expressed in MSG cells and was secreted into the cocoon of S1-V165; (2) the dimeric form of rhVEGF165 could be easily dissolved from S1-V165 cocoons using an alkaline solution; (3) rhVEGF165 extracted from S1-V165 cocoons exhibited slightly better cell proliferative activity than the hVEGF165 standard in cultured human umbilical vein endothelial cells. This study provides an alternative strategy for the production of bioactive rhVEGF165 using the MSG of transgenic silkworms.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248680	VEGF165 human			Price

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