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Expression and Purification of Recombinant Mouse Interleukin-4 and -6 From Transgenic Rice Seeds

Expression and Purification of Recombinant Mouse Interleukin-4 and -6 From Transgenic Rice Seeds

Yoshihiro Fujiwara, Lijun Yang, Fumio Takaiwa, Kenji Sekikawa

Mol Biotechnol. 2016 Apr;58(4):223-31.

PMID: 26876890

Abstract:

Transgenic rice seed can be utilized as a bioreactor to produce high-value recombinant proteins. Mouse interleukin 4 (mIL-4) and mIL-6 were specifically expressed as secretory proteins in rice endosperm by ligating the N-terminal glutelin B-1 (GluB-1) signal peptide and the C-terminal KDEL endoplasmic reticulum retention signal under control of the endosperm-specific GluB-1 promoter. In the transgenic rice seed, mIL-4 and mIL-6 accumulated in levels up to 0.43 mg/g grain and 0.16 mg/g grain, respectively. The reducing agents and detergents required for extraction from the transgenic rice seeds differed between the two proteins, indicating differences in their intracellular localization within the endosperm cell. Purified mIL-4 and mIL-6 exhibited high activity and very low endotoxin contamination.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413401	Interleukin-4 from mouse			Price

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