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Expression and Purification of the Recombinant His-tagged GST-CD38 Fusion Protein Using the baculovirus/insect Cell Expression System

Expression and Purification of the Recombinant His-tagged GST-CD38 Fusion Protein Using the baculovirus/insect Cell Expression System

Keng Meng Khoo, Chan Fong Chang, Jeffrey Schubert, Ewald Wondrak, Hiok Hee Chng

Protein Expr Purif. 2005 Apr;40(2):396-403.

PMID: 15766882

Abstract:

CD38 is a type II transmembrane glycoprotein found in myriad mammalian tissues and cell types. It is known for its involvement in the metabolism of cyclic ADP-ribose and nicotinic acid adenine dinucleotide phosphate, two nucleotides with calcium mobilizing activity independent of inositol trisphosphate. CD38 itself has been shown to have clinical significance in certain diseases with possible utilization in diagnostic and prognostic applications. Previous studies on several autoimmune diseases have shown the usefulness of recombinant CD38 protein expressed from Escherichia coli and Pichia pastoris in the detection of autoantibodies to CD38 via Western blot and ELISA. In this study, we produced a 6 x His-tagged GST-CD38 fusion protein using a recombinant baculovirus/insect cell expression technique that was purified as a soluble protein. The fusion protein was purified to homogeneity by affinity and gel filtration chromatography steps. It has an apparent molecular mass of 56 kDa on SDS-PAGE gel stained with Coomassie blue and was recognized on Western blots by antibodies against human CD38 as well as the polyhistidine tag. Peptide mass fingerprinting analysis confirmed the identity of human CD38 in the fusion protein.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42412867	CyclinC, GST tagged human			Price

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