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Expression of Human Antithrombin III in Saccharomyces Cerevisiae and Schizosaccharomyces Pombe

Expression of Human Antithrombin III in Saccharomyces Cerevisiae and Schizosaccharomyces Pombe

M Bröker, H Ragg, H E Karges

Biochim Biophys Acta. 1987 Apr 29;908(3):203-13.

PMID: 3032260

Abstract:

Recombinant plasmids were constructed that direct the synthesis of human antithrombin III in baker's yeast, Saccharomyces cerevisiae, and the fission yeast, Schizosaccharomyces pombe. The signal sequence of antithrombin III was recognized by both yeast species, and antithrombin III was secreted into the medium. When the signal sequence was replaced by a sequence of ten arbitrary amino acids, the product expressed from such a construct stayed inside the cell. Antithrombin III was glycosylated by the baker's and fission yeast and was immunologically identical to antithrombin III isolated from human plasma. Antithrombin III isolated from the culture media of recombinant yeasts was biologically active, as could be shown by progressive inhibitor activity and heparin cofactor activity.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP90170802-B	Antithrombin III from human plasma		90170-80-2	Price

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