Home
Resources
Publications
Expression of Recombinant carcinoembryonic-antigen-human-β-defensin-2 Gene in Colon Cancer HCT116 Cells

Expression of Recombinant carcinoembryonic-antigen-human-β-defensin-2 Gene in Colon Cancer HCT116 Cells

Yumei Zhang, Jianping Du, Zhengtang Chen

Cell Biochem Biophys. 2014 Apr;68(3):517-21.

PMID: 23963772

Abstract:

The objective of this study was to construct a system driving high expression of human-β-denfensin-2 (HBD-2) system in eukaryotic cell. To construct recombinant retrovirus expression vector, CEA signal peptide-HBD-2 (mature peptide sequence) was cloned in the retrovirus expression vector PLNCX2. The retroviral vector was transfected into PT67 cells by DOTAP; after screening and amplifying single clone cell by G418, the virus particles were collected and infected to eukaryotic, colon cancer HCT116 cells. Furthermore, the HCT116 cells were also screened by using G418 and the resistance clones were obtained. Finally, the expression of HBD-2 was detected by Western blotting, which suggested a high level of expression of HBD-2 in HCT116 cells. In conclusion, the results indicate that high level of HBD-2 expression was obtained in HCT116 cells which will help in effective commercial production and purification of HBD-2 protein.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42414842	Carcinoembryonic Antigen human			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: