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Expression of Soluble Recombinant TGF-beta Type II Receptor Fused With the Fc Portion of Human IgG1 (sTbetaRII-Fc) in NS0 Cells

Expression of Soluble Recombinant TGF-beta Type II Receptor Fused With the Fc Portion of Human IgG1 (sTbetaRII-Fc) in NS0 Cells

Eliza P Kwiatkowska, Urszula Kazimierczak, Andrzej Mackiewicz, Dariusz W Kowalczyk

Acta Biochim Pol. 2006;53(2):361-9.

PMID: 16733563

Abstract:

We have constructed and expressed recombinant chimeric soluble TGF-beta type II receptor fused with the Fc portion of human IgG1 (sTbetaRII-Fc) in NS0 mouse myeloma cells and isolated cell lines constitutively secreting very high levels of biologically active protein. The GS-NS0 expression system takes advantage of the strong human cytomegalovirus immediate early promoter expression vector and glutamine synthetase as a selectable marker. The recombinant chimeric receptor could be produced in high amounts and efficiently purified by one step chromatography on a protein A column. Biochemical studies revealed that recombinant sTbetaRII-Fc binds native TGF-beta1 and TGF-beta3 isoforms and neutralizes their activity in vitro.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413742	Transforming Growth Factor-β1 Receptor II Soluble Fragment human			Price

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