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Expression, Purification and Anticancer Analysis of GST-tagged Human Perforin and Granzyme B Proteins in Human Laryngeal Cancer Hep-2 Cells

Expression, Purification and Anticancer Analysis of GST-tagged Human Perforin and Granzyme B Proteins in Human Laryngeal Cancer Hep-2 Cells

Xiuying Li, Guang Zhang, Guijie An, Sha Liu, Yandong Lai

Protein Expr Purif. 2014 Mar;95:38-43.

PMID: 24291445

Abstract:

Granzyme B and perforin, two major effector molecules in the granule-mediated cytolytic pathway, are thought to be involved in suppression of tumor progression. In this study, the pGEX-4T-1 expression vector was used to express full-length human perforin or granzyme B as a GST-tagged fusion protein in Escherichia coli (E. coli). GST-tagged proteins were induced with IPTG and purified by GSTrap 4B columns. Purified fusion proteins migrated at the predicted molecular mass on SDS-PAGE and were recognized by specific antibodies. Moreover, the fusion proteins can induce apoptosis and directly inhibit the growth of human laryngeal cancer Hep-2 cells in vitro. These results suggest that active perforin and granzyme B fusion proteins can be produced in E. coli and exhibit anticancer potential in laryngeal cancer cells.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411820	CAMK1δ, active, GST tagged human			Price
IAR42415012	P73b, GST tagged human			Price

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