Home
Resources
Publications
Extraction, Characterization and Biological Activity of a (1,3)(1,6)-β-d-glucan From the Pathogenic Oomycete Pythium Insidiosum

Extraction, Characterization and Biological Activity of a (1,3)(1,6)-β-d-glucan From the Pathogenic Oomycete Pythium Insidiosum

Juliana S M Tondolo, Pauline C Ledur, Érico S Loreto, Camila M Verdi, Paula E R Bitencourt, Francielli P K de Jesus, Jean P Rocha, Sydney H Alves, Guilherme L Sassaki, Janio M Santurio

Carbohydr Polym. 2017 Feb 10;157:719-727.

PMID: 27987983

Abstract:

Pythiosis is a life-threatening infectious disease caused by the pathogenic oomycete Pythium insidiosum. This study is the first to evaluate the P. insidiosum glucan content and its biological activities. The enzymatic quantification of the glucans in P. insidiosum mycelia showed that the β-glucan content was 18.99%±3.59. The cell wall polysaccharide extract consisted of ∼81.7% carbohydrates (exclusively glucose) and ∼18.3% residual amino acids and peptides. The results from monosaccharide composition, methylation and 1D/2D NMR spectroscopy analyses indicated the presence of a highly branched (1,3)(1,6)-β-d-glucan, with (1,6)-β-d-glucopyranosil side-branching unit on average every 1-2 repeat units. In vitro, the β-d-glucan extract could significantly promote spleen lymphocyte proliferation in human, equine and mouse cell cultures. BALB/c mice that were subcutaneously pre-immunized with three doses of 0.5, 2.5 and 5.0mg of β-glucan/mouse, showed a significant increase in IL-2, IL-6, IL-10, TNF-α and IL-17A production compared to non-immunized mice. These results suggested that β-d-glucan extract induces significant and specific Th17 cellular immune response and provided the theoretical basis for further experiments.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP22387037	Dipotassium deuterium phosphate		22387-03-7	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: