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Facile SPS of Peptides Having C-terminal Asn and Gln

G Breipohl, J Knolle, W Stüber

Int J Pept Protein Res. 1990 Mar;35(3):281-3.

PMID: 2354879

Abstract:

Attachment of Fmoc-asparagine or glutamine to p-alkoxybenzyl alcohol type resins has always been difficult and not very effective. A very simple and effective method for the preparation of peptides terminating in asparagine or glutamine is described. The method involves quantitative attachment of Fmoc-Asp-OtBu or Fmoc-Glu-OtBu via their side-chain carboxyl group to a resin functionalized with our TMBPA linker for peptide amides. Peptide synthesis is performed using our standard Fmoc chemistry, and treatment with acid, e.g. TFA/DCM/scavenger mixtures, releases the Asn or Gln peptides.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42410621 Fmoc-Glu(OtBu)-OH-15N Fmoc-Glu(OtBu)-OH-15N Price
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