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Fecal and Urinary NMR-based Metabolomics Unveil an Aging Signature in Mice

Fecal and Urinary NMR-based Metabolomics Unveil an Aging Signature in Mice

Riccardo Calvani, Elisa Brasili, Giulia Praticò, Giorgio Capuani, Alberta Tomassini, Federico Marini, Fabio Sciubba, Alberto Finamore, Marianna Roselli, Emanuele Marzetti, Alfredo Miccheli

Exp Gerontol. 2014 Jan;49:5-11.

PMID: 24184118

Abstract:

Background:
Aging is characterized by derangements in multiple metabolic pathways that progressively constrict the homeostatic reserve (homeostenosis). The signature of metabolic alterations that accompany aging can be retrieved through the metabolomic profiling of biological fluids.
Objective:
To characterize the age-related changes in urinary and fecal metabolic profiles of BALB/c mice through a (1)H nuclear magnetic resonance (NMR)-based metabolomic approach.
Methods:
Young (n=19) and old (n=13) male BALB/c mice were fed ad libitum standard laboratory chow. Twenty four-hour feces and urine were collected using metabolic cages and analyzed by high-resolution (1)H NMR spectroscopy combined with multivariate statistical analyses.
Results:
An age-related metabolic phenotype was detected both in urine and feces. The metabolic signature of aging consisted of changes in levels of metabolites associated with amino acid metabolism, tricarboxylic acid cycle, tryptophan-nicotinamide adenine dinucleotide pathway, and host-microbiota metabolic axis.
Conclusions:
Our (1)H NMR-based metabolomic approach was able to characterize the effect of age on urinary and fecal metabotypes. The implementation of this analytical strategy may increase our understanding of the metabolic alterations involved in the aging process and assist in the design of anti-aging interventions.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1105675624	5-Methoxy-N-phenyl-4-(trimethylsilyl)nicotinamide		1105675-62-4	Price

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