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Fed-Batch CHO Cell Culture for Lab-Scale Antibody Production

Yuzhou Fan, Daniel Ley, Mikael Rørdam Andersen

Methods Mol Biol. 2018;1674:147-161.

PMID: 28921435

Abstract:

Fed-batch culture is the most commonly used upstream process in industry today for recombinant monoclonal antibody production using Chinese hamster ovary (CHO) cells. Developing and optimizing this process in the lab is crucial for establishing process knowledge, which enables rapid and predictable tech-transfer to manufacturing scale. In this chapter, we describe stepwise how to carry out fed-batch CHO cell culture for lab-scale antibody production.

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