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Fermentation Product Butane 2,3-diol Induces Ca2+ Transients in E. Coli Through Activation of Lanthanum-Sensitive Ca2+ Channels

Fermentation Product Butane 2,3-diol Induces Ca2+ Transients in E. Coli Through Activation of Lanthanum-Sensitive Ca2+ Channels

Anthony K Campbell, Riffat Naseem, Kenneth Wann, I Barry Holland, Stephanie B Matthews

Cell Calcium. 2007 Feb;41(2):97-106.

PMID: 16842848

Abstract:

The results here are the first demonstration of a physiological agonist opening Ca2+ channels in bacteria. Bacteria in the gut ferment glucose and other substrates, producing alcohols, diols, ketones and acids, that play a key role in lactose intolerance, through the activation of Ca2+ and other ion channels in host cells and neighbouring bacteria. Here we show butane 2,3-diol (5-200mM; half maximum 25mM) activates Ca2+ transients in E. coli, monitored by aequorin. Ca2+-transient magnitude depended on external Ca2+ (0.1-10mM). meso-Butane 2,3-diol was approximately twice as potent as 2R,3R (-) and 2S,3S (+) butane 2,3-diol. There were no detectable effects on cytosolic free Ca2+ of butane 1,3-diol, butane 1,4-diol and ethylene glycol. The glycerol fermentation product propane 1,3-diol only induced significant Ca2+ transients in 10mM external Ca2. Ca2+ butane 2,3-diol Ca2+ transients were due to activation of Ca2+ influx, followed by activation of Ca2+ efflux. The effect of butane 2,3-diol was abolished by La3+, and markedly reduced as a function of growth phase. These results were consistent with butane 2,3-diol activating a novel La3+-sensitive Ca2+ channel. They have important implications for the role of butane 2,3-diol and Ca2+ in bacterial-host cell signalling.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP107880-B	Butane-1,3-diol		107-88-0	Price

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