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Feulgen-Naphthol Yellow S Cytophotometry of Liver Cells. The Effect of Formaldehyde Induced Shrinkage on Nuclear Naphthol Yellow S Binding

Feulgen-Naphthol Yellow S Cytophotometry of Liver Cells. The Effect of Formaldehyde Induced Shrinkage on Nuclear Naphthol Yellow S Binding

J Gaub

Histochemistry. 1976 Nov 12;49(4):293-301.

PMID: 62740

Abstract:

1. In isolated liver cells, fixed in 4 per cent formaldehyde (NFS) for Feulgen-Naphthol Yellow S (F-NYS) staining of DNA and protein, nuclear shrinkage increases the nuclear concentration of solids to 46 per cent (w/v) before the start of the NYS staining. 2. When a fixative mixture of methanol:acetic acid:formalin (85:5:10 by volume; MAF) is used, the concentration of nuclear solids during NYS staining remain at a physiological level of 19 per cent. 3. By exposing liver cells to NFS for 10 to 120 seconds before fixation in MAF, increasing nuclear shrinkage can be induced with increasing pretreatment in NFS. Nuclear NYS binding decreases in parallel with the decreasing nuclear volume in cells thus treated. As the shrinkage induced reduction in NYS binding may vary with the net charge of nuclear non-histone proteins, MAF fixation must be preferred for quantitative determinations of nuclear non-histone protein in F-NYS stained, isolated cells. 4. Fixation in MAF offers the same advantages as NFS fixation as regards the small loss of proteins during the Feulgen staining procedure and the excellent reproducibility of the F-NYS staining. Storage of MAF fixed cells in the fixative for a few days does not alter their F-NYS staining properties. 5. In MAF fixed, F-NYS stained cells there is no NYS binding to histone basic amino acid residues.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP846708	Naphthol Yellow S		846-70-8	Price

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