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Fine Specificities of Two Lectins From Cymbosema Roseum Seeds: A Lectin Specific for High-Mannose Oligosaccharides and a Lectin Specific for Blood Group H Type II Trisaccharide

Fine Specificities of Two Lectins From Cymbosema Roseum Seeds: A Lectin Specific for High-Mannose Oligosaccharides and a Lectin Specific for Blood Group H Type II Trisaccharide

Tarun K Dam, Benildo S Cavada, Celso S Nagano, Bruno Am Rocha, Raquel G Benevides, Kyria S Nascimento, Luiz Ag de Sousa, Stefan Oscarson, C Fred Brewer

Glycobiology. 2011 Jul;21(7):925-33.

PMID: 21406562

Abstract:

The legume species of Cymbosema roseum of Diocleinae subtribe produce at least two different seed lectins. The present study demonstrates that C. roseum lectin I (CRL I) binds with high affinity to the "core" trimannoside of N-linked oligosaccharides. Cymbosema roseum lectin II (CRL II), on the other hand, binds with high affinity to the blood group H trisaccharide (Fucα1,2Galα1-4GlcNAc-). Thermodynamic and hemagglutination inhibition studies reveal the fine binding specificities of the two lectins. Data obtained with a complete set of monodeoxy analogs of the core trimannoside indicate that CRL I recognizes the 3-, 4- and 6-hydroxyl groups of the α(1,6) Man residue, the 3- and 4-hydroxyl group of the α(1,3) Man residue and the 2- and 4-hydroxyl groups of the central Man residue of the trimannoside. CRL I possesses enhanced affinities for the Man5 oligomannose glycan and a biantennary complex glycan as well as glycoproteins containing high-mannose glycans. On the other hand, CRL II distinguishes the blood group H type II epitope from the Lewis(x), Lewis(y), Lewis(a) and Lewis(b) epitopes. CRL II also distinguishes between blood group H type II and type I trisaccharides. CRL I and CRL II, respectively, possess differences in fine specificities when compared with other reported mannose and fucose recognizing lectins. This is the first report of a mannose-specific lectin (CRL I) and a blood group H type II-specific lectin (CRL II) from seeds of a member of the Diocleinae subtribe.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42410783	H-Trisaccharide			Price

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