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Five Treatment Procedures Evaluated for the Elimination of Ascorbate Interference in the Enzymatic Determination of Urinary Oxalate

Five Treatment Procedures Evaluated for the Elimination of Ascorbate Interference in the Enzymatic Determination of Urinary Oxalate

K V Inamdar, K G Raghavan, D S Pradhan

Clin Chem. 1991 Jun;37(6):864-8.

PMID: 2049851

Abstract:

We evaluated the efficacies of five treatment procedures for eliminating ascorbate interference in the enzymatic determination of urinary oxalate. Aliquots of urine samples, containing different amounts of added ascorbate and oxalate, were individually subjected to ferric chloride, sodium nitrite, sodium periodate, charcoal, or ascorbate oxidase treatment to eliminate ascorbate interference. Oxalate contents of the urine samples were then determined by a banana oxalate oxidase-horseradish peroxidase-linked assay with 3-methyl-2-benzothiazolinone hydrazone and 3-(dimethylamino)benzoic acid as chromogens. Only those urine samples treated with ascorbate oxidase or charcoal consistently gave recovery of oxalate close to 100%. Treatment with other reagents, though improving the recovery of oxalate, gave inconsistent results. On the basis of these data, we describe procedures for simply and reliably assaying oxalate by using banana oxalate oxidase.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP99649	3-(Dimethylamino)benzoic acid		99-64-9	Price

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