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Flow Cytometric Analysis on Cytotoxic Action of Amyloid Beta Protein Fragment 25-35 on Brain Neurons Dissociated From the Rats

Flow Cytometric Analysis on Cytotoxic Action of Amyloid Beta Protein Fragment 25-35 on Brain Neurons Dissociated From the Rats

K Furukawa, Y Oyama, L Chikahisa, Y Hatakeyama, N Akaike

Brain Res. 1994 Oct 31;662(1-2):259-62.

PMID: 7859081

Abstract:

Effects of amyloid beta protein fragment 25-35, A beta P(25-35), on membrane permeability and cell viability were examined in the brain neurons dissociated from the rats using a flow cytometer and two fluorescent dyes, fluo-3 to monitor intracellular Ca2+ concentration ([Ca2+]i) of neurons and ethidium which is impermeant to membranes of intact neurons to stain dead and dying neurons. A beta P(25-35) augmented fluo-3 fluorescence of some neurons at concentrations greater than 1 microM, indicating an increase in [Ca2+]i although other neurons (about 80% of total neurons) did not respond to A beta P(25-35) even at 10 microM. A beta P(25-35) at 1 microM or greater increased dose-dependently the number of ethidium-stained neurons, suggesting a dose-dependent increase in number of dead and dying neurons. Results suggest that A beta P(25-35) increases the membrane permeability of brain neurons, resulting in a destabilized intracellular homeostasis that leads to neuonal death.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP131602534	Amyloid β-Protein Fragment 25-35		131602-53-4	Price

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