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Fluorescein Diacetate Microplate Assay in Cell Viability Detection

Fluorescein Diacetate Microplate Assay in Cell Viability Detection

Xi Chen, Xiu-Ying Yang, Lian-Hua Fang, Guan-Hua DU

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2016 Dec 20;38(6):710-714.

PMID: 28065239

Abstract:

Objective To investigate the application of the fluorescein diacetate (FDA) microplate assay in cell viability detection. Methods Cells were seeded in a 96-well culture plate until detection. After incubated with FDA,the plate was detected by fluorescence microplate analyzer. The effects of FDA incubation duration,concentration,and other factors on the assay's accuracy and stability were assessed. We also compared the results of FDA with methyl thiazolyl(MTT) in terms of cell numbers and H2O2 injury. Results Within 0-30 minutes,the fluorescence-cell number coefficient of FDA assay increased with duration and reached 0.99 in 27-30 minutes. The optimum concentration of final FDA in this study was 10-30 μg/ml. On cell viability detection,the result of FDA method was equivalent to MTT method. As to H2O2 injury assay,the sensitivity of FDA method was superior to MTT on the higher concentration H2O2 treatment due to a relative shorter duration for detection. Conclusion As a stable and reliable method,FDA is feasible for cell variability detection under varied conditions.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP596098-B	Fluorescein diacetate		596-09-8	Price

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