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Fluorescence Competition Assay for the Assessment of Green Leaf Volatiles and trans-β-farnesene Bound to Three Odorant-Binding Proteins in the Wheat Aphid Sitobion Avenae (Fabricius)

Fluorescence Competition Assay for the Assessment of Green Leaf Volatiles and trans-β-farnesene Bound to Three Odorant-Binding Proteins in the Wheat Aphid Sitobion Avenae (Fabricius)

Tao Zhong, Jiao Yin, Sisi Deng, Kebin Li, Yazhong Cao

J Insect Physiol. 2012 Jun;58(6):771-81.

PMID: 22306433

Abstract:

Odorant-binding proteins (OBPs) are important parts of insect olfactory systems, and sensitive olfaction is vital for phytophagous insects in host foraging. Electrophysiological studies are helpful in understanding olfactory sensing in Sitobion avenae (Hemiptera: Aphididae), but the functions of odorant-binding proteins in this insect are poorly understood. In this study, we used fluorescence competition assays to measure the binding specificities of SaveOBPs. The results showed that both SaveOBP2 and SaveOBP3 were superior to SaveOBP7 in binding green leaf volatiles. It was unexpected that SaveOBP7 bound trans-β-farnesene strongly, which was known as alarm pheromone of this species. Host volatiles were recognized much more easily by SaveOBP2, and the observed binding activity of SaveOBP2 equaled for tested green leaf volatiles. Our results imply that SaveOBP7 might play a more important role in aphid alarm pheromone discrimination.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP18794848	trans-β-Farnesene		18794-84-8	Price

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