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Fluorescent Derivatization of Xanthurenic Acid and Nicotinic Acid With Horseradish Peroxidase in the Presence of Excess Hydrogen Peroxide

Fluorescent Derivatization of Xanthurenic Acid and Nicotinic Acid With Horseradish Peroxidase in the Presence of Excess Hydrogen Peroxide

Junichi Odo, Yuto Sogawa, Masahiko Inoguchi, Akihito Hirai

Anal Sci. 2011;27(1):105-9.

PMID: 21233570

Abstract:

The fluorescent derivatization of tryptophan metabolites (xanthurenic acid, nicotinic acid, picolinic acid, and 3-hydroxyanthranilic acid) by the catalytic activity of horseradish peroxidase (HRP) was investigated in the presence of excess H(2)O(2). Non-fluorescent xanthurenic acid (XA) and nicotinic acid (NA) were converted into a fluorescent compound with maximum excitation and emission wavelengths at 325 and 425 nm, and 318 and 380 nm, respectively. This fluorescent derivatization was developed for the fluorometric determination of trace amounts of XA and NA. The calibration curves were linear from 1.0 to 10.0 nmol XA and from 5.0 to 20.0 nmol NA in a 1.0-mL sample solution. The UV spectra of the reaction solutions suggested that compound III as an intermediate of HRP played an essential role in this fluorescent derivatization with HRP.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1189954797	Nicotinic acid-13C6 solution		1189954-79-7	Price

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