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Fluorimetric Detection of Reserpine in Mouse Serum Through Online Post-Column Electrochemical Derivatization

Ning Chen, Weixia Li, Shuchao Wu, Yan Zhu

R Soc Open Sci. 2018 Aug 15;5(8):171948.

PMID: 30224984

Abstract:

A novel method combining high-performance liquid chromatography with online post-column electrochemical derivatization and fluorescence detection was established for the detection of reserpine in mouse serum. Reserpine separation was conducted using a C18 column with 5 mM H3PO4 and acetonitrile (55/45, v/v) as eluent. Reserpine was then electro-oxidized into a strongly fluorescent compound using an electrolytic cell device. Detection parameters, such as potential and fluorescence wavelength, were optimized. The linearity of the proposed method ranged from 0.01 to 5.0 mg l-1 with a correlation coefficient of 0.9997. The limit of qualification (S/N = 10) and limit of detection (S/N = 3) were 9.7 and 2.9 µg l-1, respectively. Resperine recoveries from spiked blank and drug-treated mouse serum samples ranged from 92.0 to 115%.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP84759115 Reserpine-(3′,4′,5′-trimethoxy-d9) Reserpine-(3′,4′,5′-trimethoxy-d9) 84759-11-5 Price
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