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Fluorometric 'Switch-On' Detection of Heparin Based on a System Composed of Rhodamine-Labeled Chitosan Oligosaccharide Lactate, and Graphene Oxide

Fluorometric 'Switch-On' Detection of Heparin Based on a System Composed of Rhodamine-Labeled Chitosan Oligosaccharide Lactate, and Graphene Oxide

Linlin Zhong, Kyusik Yun

Methods Appl Fluoresc. 2018 May 29;6(3):035011.

PMID: 29765011

Abstract:

A novel fluorescence 'Switch on' for the detection of heparin based on the RhB-COL/GO system was achieved. A strong fluorescence dye, Rhodamine B, was modified by chitosan oligosaccharide lactate (COL), which plays a major role in the formation of a positively charged RhB-COL complex. RhB-COL was soluble and stable in solution, which was characterized by using Fourier transform infrared spectroscopy and x-ray photoelectron spectroscopy. GO sheets quenched the fluorescence intensity of RhB-COL due to electron transfer from RhB to the GO surface. The decrease in fluorescence intensity of RhB-COL with increasing GO concentration was recorded using a Cary Eclipse fluorescence spectrophotometer. On the other hand, the addition of heparin replaced GO to bind with the RhB-COL surface via an electrostatic and noncovalent bond due to the abundant negative charge, which resulted in recovery of the fluorescence intensity. This RhB-COL/GO system possessed high selectivity and good sensitivity for the detection of heparin compared to other biomolecules, such as glycine, D-glucose, hyaluronic acid, L-glutamic acid, and ascorbic acid. The linear response toward heparin was measured over the range, 0-1.8 U · ml-1, with a low detection limit of 0.04 U · ml-1. The satisfactory sensing performance of RhB-COL/GO for heparin supports new 'switch-on' sensor applications in heparin-related biomedical detection.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP148411578	Chitosan oligosaccharide lactate		148411-57-8	Price

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