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Formaldehyde Crosslinking: A Tool for the Study of Chromatin Complexes

Formaldehyde Crosslinking: A Tool for the Study of Chromatin Complexes

Elizabeth A Hoffman, Brian L Frey, Lloyd M Smith, David T Auble

J Biol Chem. 2015 Oct 30;290(44):26404-11.

PMID: 26354429

Abstract:

Formaldehyde has been used for decades to probe macromolecular structure and function and to trap complexes, cells, and tissues for further analysis. Formaldehyde crosslinking is routinely employed for detection and quantification of protein-DNA interactions, interactions between chromatin proteins, and interactions between distal segments of the chromatin fiber. Despite widespread use and a rich biochemical literature, important aspects of formaldehyde behavior in cells have not been well described. Here, we highlight features of formaldehyde chemistry relevant to its use in analyses of chromatin complexes, focusing on how its properties may influence studies of chromatin structure and function.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP86356732	Formaldehyde O-pentafluorophenylmethyl-oxime		86356-73-2	Price

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