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Formation of 10-formylfolic Acid, a Potent Inhibitor of Dihydrofolate Reductase, in Rat Liver Slices Incubated With Folic Acid

M D'Urso-Scott, J Uhoch, J R Bertino

Proc Natl Acad Sci U S A. 1974 Jul;71(7):2736-9.

PMID: 4527808

Abstract:

During investigation of folate polyglutamate biosynthesis in rat liver slices utilizing [2-(14)C]folic acid, a folate compound that behaved like a polyglutamate form in the Sephadex G-15 gel filtration system was found to accumulate. Subsequent chromatographic, spectral, chemical, and enzymic studies have indicated that the compound formed in liver slices incubated with [(14)C]folic acid with and without methotrexate was 10-formyl folate. This folate is of interest in that it is the most potent natural inhibitor of dihydrofolate reductase known and may be capable of serving a regulatory function within the cell.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP134054 10-Formylfolic acid 10-Formylfolic acid 134-05-4 Price
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