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Frameshift Mutations Produced by 9-aminoacridine in Wild-Type, uvrA and recA Strains of Escherichia Coli; Specificity Within a Hotspot

Frameshift Mutations Produced by 9-aminoacridine in Wild-Type, uvrA and recA Strains of Escherichia Coli; Specificity Within a Hotspot

Niranjan Acharya, Nagla Fathi Abu-Nasr, Genta Kawaguchi, Masaru Imai, Kazuo Yamamoto

J Radiat Res. 2007 Sep;48(5):361-8.

PMID: 17611351

Abstract:

The endogenous tonB gene of Escherichia coli was used as a target for 9-aminoacridine-induced mutations that were identified in recA(-) and uvrA(-) cells. The cytotoxicity of 9-aminoacridine was enhanced in the uvrA and recA strains compared to the wild-type strain, and the mutagenicity of 9-aminoacridine in the uvrA and recA strains was similar to that in the wild type. For all three strains, the most common mutations were minus frameshifts in repetitive G:C base-pairs followed by minus frameshifts in nonrepetitive G:C base-pairs. 9-aminoacridine-induced minus frameshifts in the wild-type strain were distributed with several hot and warm spots. These sites were also hot and warm spots for minus frameshifts in the recA and uvrA stains. Furthermore, they were hot and warm sites in a 9-aminoacridine-treated strain carrying the target tonB gene oriented in the opposite direction. 9-Aminoacridine is known to interact with DNA to form intercalations which are involved in minus frameshift mutagenesis. In this study, we therefore argue that 1) 9-aminoacridine can induce bulky DNA lesions which are excised by nucleotide excision repair and not involved in mutagenesis, 2) the presence or absence of a recA-dependent repair pathway does not influence the mutagenic effect of 9-aminoacridine, and 3) both leading strand and lagging strand replication equally produce minus frameshifts, therefore gene orientation is not an important determinant of the formation of hot and warm spots by 9-aminoacridine.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP90459	9-Aminoacridine		90-45-9	Price

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