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Functional Characterization of Fluorescent Hepcidin

Functional Characterization of Fluorescent Hepcidin

Franz Dürrenberger, Vincenzo Abbate, Yongmin Ma, Maria C Arno, Dareen Jaiash, Archna Parmar, Victoria Marshall, Gladys O Latunde-Dada, Tina Zimmermann, David Senn, Patrick Altermatt, Vania Manolova, Robert C Hider, etc.

Bioconjug Chem. 2013 Sep 18;24(9):1527-32.

PMID: 23888876

Abstract:

Hepcidin is a peptide hormone that regulates homeostasis in iron metabolism. It binds to the sole known cellular iron exporter ferroportin (Fpn), triggers its internalization, and thereby modulates the efflux of iron from cells. This functional property has been adopted in this study to assess the bioactivity and potency of a range of novel fluorescent hepcidin analogues. Hepcidin was selectively labeled with 6-carboxyfluorescein (CF) and 6-carboxytetramethylrhodamine (TMR) using Fmoc solid phase peptide chemistry. Internalization of Fpn by hepcidin was assessed by high-content microscopic analysis. Both K18- and M21K-labeled hepcidin with TMR and CF exhibited measurable potency when tested in cultured MDCK and T47D cells expressing human ferroportin. The bioactivity of the labeled hepcidin varies with the type of fluorophore and site of attachment of the fluorophores on the hepcidin molecule.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP91809675	6-Carboxytetramethylrhodamine		91809-67-5	Price

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