Home
Resources
Publications
Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments

Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments

Qingsu Cheng, Ashwin Aravind, Matthew Buckley, Andrew Gifford, Bahram Parvin

Sci Rep. 2015 Sep 8;5:13685.

PMID: 26347365

Abstract:

To date, in situ visualization of microbial density has remained an open problem. Here, functionalized buckyballs (e.g., C60-pyrrolidine tris acid) are shown to be a versatile platform that allows internalization within a microorganism without either adhering to the cell wall and cell membrane or binding to a matrix substrate such as soil. These molecular probes are validated via multi-scale imaging, to show association with microorganisms via fluorescence microscopy, positive cellular uptake via electron microscopy, and non-specific binding to the substrates through a combination of fluorescence and autoradiography imaging. We also demonstrate that cysteine-functionalized C60-pyrrolidine tris acid can differentiate live and dead microorganisms.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS76133	C60 Pyrrolidine tris-acid			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: