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Further Characterization of Carboxymethylated Aspartic Acid Agarose. Purification of Human Alpha 2-macroglobulin and Hemopexin

T Mantovaara, H Pertoft, J Porath

Biotechnol Appl Biochem. 1991 Jun;13(3):371-9.

PMID: 1715716

Abstract:

alpha 2-Macroglobulin and hemopexin were purified by affinity chromatography on a recently introduced chelating matrix, i.e., carboxymethylated aspartic acid agarose, coupled with cobalt(II). Adsorption was performed at neutral pH and the proteins were eluted by lowering the pH to 5.0. An alternative method for desorption as well as comparison with iminodiacetic acid agarose coupled with cobalt(II) is also described.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42410971 Iminodiacetic acid Agarose Iminodiacetic acid Agarose Price
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