0

[Gas Chromatographic Determination of Phenytoin, Phenobarbital and Primidone: Flash-Methylation After Direct Addition of Trimethyl-Phenyl-Ammonium Hydroxide to the Ethyl Acetate Extract (Author's Transl)]

G Brügmann

J Clin Chem Clin Biochem. 1981 May;19(5):305-6.

PMID: 7264573

Abstract:

25 microliters plasma, 25 microliters internal standard and 5 microliters sodium dihydrogenphosphate solution are extracted with 100 microliters ethyl acetate. 40 microliters of the extract are mixed with 10 microliters trimethyl-phenyl-ammoniumhydroxide (0.1 mol/l methanol). 3 microliters of this solution are injected for gas chromatography using a nitrogen-specific detector. Trimethyl-phenyl-ammoniumacetate appears to be a satisfactory methylating agent for these drugs in the injection port of the gas chromatograph.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP1899021 Trimethylphenylammonium hydroxide solution Trimethylphenylammonium hydroxide solution 1899-02-1 Price
qrcode