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Gas Chromatographic-Mass Spectrometric Determination of 11-dehydrothromboxane B2 in Human Urine

Gas Chromatographic-Mass Spectrometric Determination of 11-dehydrothromboxane B2 in Human Urine

C Weber, J Beetens, F De Clerck, F Tegtmeier

J Chromatogr. 1992 May 20;577(1):1-7.

PMID: 1400727

Abstract:

The extension of a method for the determination of thromboxane B2 (TxB2), 2,3-dinor-TxB2, 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) and 2,3-dinor-6-keto-PGF1 alpha to quantify 11-dehydro-TxB2 in the same urinary sample is described. After phenylboronic acid and C18 column chromatography, 11-dehydro-TxB2, which is present in urine as the lactone and its corresponding hydroxy acid, was quantitatively converted into its lactone form for a thin-layer purification step and pentafluorobenzyl esterification. Quantification of eicosanoids was achieved by analysing their trimethylsilyl ethers with gas chromatography and negative-ion chemical ionization mass spectrometry. The overall recovery from urine for tritiated 11-dehydro-TxB2 was 80%. The detection limit was 10 pg/ml. The method was applied to the determination of these eicosanoids in volunteers and in patients suffering from acute myocardial infarction.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP177171163	3-(Trimethylsilyl)phenylboronic acid		177171-16-3	Price

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