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GC-MS Quantification of Urinary Symmetric Dimethylarginine (SDMA), a Whole-Body Symmetric L-Arginine Methylation Index

GC-MS Quantification of Urinary Symmetric Dimethylarginine (SDMA), a Whole-Body Symmetric L-Arginine Methylation Index

Alexander Bollenbach, Erik Hanff, Bibiana Beckmann, Ruan Kruger, Dimitrios Tsikas

Anal Biochem. 2018 Sep 1;556:40-44.

PMID: 29944873

Abstract:

Circulating and excretory NG,N´G-dimethyl-l-arginine (symmetric dimethylarginine, SDMA) and NG,NG-dimethyl-l-arginine (asymmetric dimethylarginine, ADMA) are cardiovascular risk factors. Despite close chemical structures, the gas chromatography-mass spectrometry (GC-MS) measurement of SDMA is remarkably more difficult than that of ADMA for as yet unknown reasons. Here, we describe an improved GC-MS method for the quantitative determination of SDMA in human urine using commercially available NG,N´G-di-[2H3]methyl-l-arginine (d6-SDMA) as internal standard. The method is based on a single derivatization step with pentafluoropropionic anhydride (PFPA) in ethyl acetate (30 min, 65 °C) to N,N,N,O-tetrakis-pentafluoropropionyl derivatives, electron-capture negative-ion chemical ionization and selected-ion monitoring of the mass-to-charge (m/z) ions of m/z 456 for SDMA and m/z 462 for d6-SDMA.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP356423	Pentafluoropropionic anhydride		356-42-3	Price

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