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Gelsolin Bound β-amyloid peptides(1-40/1-42): Electrochemical Evaluation of Levels of Soluble Peptide Associated With Alzheimer's Disease

Gelsolin Bound β-amyloid peptides(1-40/1-42): Electrochemical Evaluation of Levels of Soluble Peptide Associated With Alzheimer's Disease

Yanyan Yu, Xiaoyu Sun, Daoquan Tang, Chenglin Li, Lin Zhang, Dongxia Nie, Xiaoxing Yin, Guoyue Shi

Biosens Bioelectron. 2015 Jun 15;68:115-121.

PMID: 25562737

Abstract:

A method for the highly sensitive determination of soluable β-amyloid peptides (Aβ(1-40/1-42)) that employs a detection bioconjugate of HRP-Au-gelsolin as the electrochemical nanoprobe is presented. Contrary to previous detection notions that utilized antibodies, which could specifically recognize the N- or C-terminus of peptides, we demonstrate herein that the reported specific binding between gelsolin and Aβ might provide an alternative way to evaluate the peptides sensitively and selectively. The HRP-Au-gelsolin nanohybrid was designed by one-pot functionalization of Au nanaoparticles (NPs) with horseradish peroxidase (HRP) and gelsolin. Through a sandwich-type sensor array, soluble Aβ(1-40/1-42) were captured onto the array due to the interactions between targeted peptides and surface-confined gelsolin and electrochemical signals were amplified by abundant attachments of HRP labeled on AuNPs, which could specifically catalyse its substrate, 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to give rise to measurable signals. The proposed gelsolin-bound Aβ methodology displayed satisfactory sensitivity and wide linear range towards Aβ(1-40/1-42) with a detection limit down to 28 pM, which are verified to be sensitive-enough for the assessment of Aβ levels both in normal and Alzheimer's disease (AD) rat brains. Experimental results indicated that compared with normal group, soluble β-amyloid peptide levels in cerebrospinal fluid (CSF) and targeted brain tissues of AD rats all declined with differentiable degrees. In short, the newly unfolding strategy presents valuable information related to pathological events in brain and will exhibit a braw perspective for the early diagnosis of AD process.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP107761422-B	β-Amyloid Peptide (1-42), Rat		107761-42-2	Price

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