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Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix Espanaensis

Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix Espanaensis

Martin Berner, Daniel Krug, Corina Bihlmaier, Andreas Vente, Rolf Müller, Andreas Bechthold

J Bacteriol. 2006 Apr;188(7):2666-73.

PMID: 16547054

Abstract:

The saccharomicins A and B, produced by the actinomycete Saccharothrix espanaensis, are oligosaccharide antibiotics. They consist of 17 monosaccharide units and the unique aglycon N-(m,p-dihydroxycinnamoyl)taurine. To investigate candidate genes responsible for the formation of trans-m,p-dihydroxycinnamic acid (caffeic acid) as part of the saccharomicin aglycon, gene expression experiments were carried out in Streptomyces fradiae XKS. It is shown that the biosynthetic pathway for trans-caffeic acid proceeds from L-tyrosine via trans-p-coumaric acid directly to trans-caffeic acid, since heterologous expression of sam8, encoding a tyrosine ammonia-lyase, led to the production of trans-p-hydroxycinnamic acid (coumaric acid), and coexpression of sam8 and sam5, the latter encoding a 4-coumarate 3-hydroxylase, led to the production of trans-m,p-dihydroxycinnamic acid. This is not in accordance with the general phenylpropanoid pathway in plants, where trans-p-coumaric acid is first activated before the 3-hydroxylation of its ring takes place.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP501166	trans-Caffeic acid		501-16-6	Price

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