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Genetic and Antigenic Characteristics of ApxIIA and ApxIIIA From Actinobacillus Pleuropneumoniae Serovars 2, 3, 4, 6, 8 and 15

Genetic and Antigenic Characteristics of ApxIIA and ApxIIIA From Actinobacillus Pleuropneumoniae Serovars 2, 3, 4, 6, 8 and 15

Ho To, Shinya Nagai, Akira Iwata, Tomohiro Koyama, Atsushi Oshima, Nobuyuki Tsutsumi

Microbiol Immunol. 2016 Jul;60(7):447-58.

PMID: 27211905

Abstract:

Apx toxins produced by Actinobacillus pleuropneumoniae are essential components of new generation vaccines. In this study, apxIIA and apxIIIA genes of serovars 2, 3, 4, 6, 8 and 15 were cloned and sequenced. Amino acid sequences of ApxIIA proteins of serovars 2, 3, 4, 6, 8 and 15 were almost identical to those of serovars 1, 5, 7, 9 and 11-13. Immunoblot analysis showed that rApxIIA from serovars 2 and 15 reacts strongly with sera from animals infected with various serovars. Sequence analysis revealed that ApxIIIA proteins has two variants, one in strains of serovar 2 and the other in strains of serovars 3, 4, 6, 8 and 15. A mouse cross-protection study showed that mice actively immunized with rApxIIIA/2 or rApxIIIA/15 are protected against challenge with A. pleuropneumoniae strains of serovars 3, 4, 6, 8, 15, and 2 expressing ApxIII/15 and ApxIII/2, respectively. Similarly, mice passively immunized with rabbit anti-rApxIIIA/2 or anti-rApxIIIA/15 sera were found to be protected against challenge with strains of serovars 2 and 15. Our study revealed antigenic and sequence similarities within ApxIIA and ApxIIIA proteins, which may help in the development of effective vaccines against disease caused by A. pleuropneumoniae.

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