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Genetic Lineage Tracing Identifies in Situ Kit-expressing Cardiomyocytes

Genetic Lineage Tracing Identifies in Situ Kit-expressing Cardiomyocytes

Qiaozhen Liu, Rui Yang, Xiuzhen Huang, Hui Zhang, Lingjuan He, Libo Zhang, Xueying Tian, Yu Nie, Shengshou Hu, Yan Yan, Li Zhang, Zengyong Qiao, Qing-Dong Wang, Kathy O Lui, Bin Zhou

Cell Res. 2016 Jan;26(1):119-30.

PMID: 26634606

Abstract:

Cardiac cells marked by c-Kit or Kit, dubbed cardiac stem cells (CSCs), are in clinical trials to investigate their ability to stimulate cardiac regeneration and repair. These studies were initially motivated by the purported cardiogenic activity of these cells. Recent lineage tracing studies using Kit promoter to drive expression of the inducible Cre recombinase showed that these CSCs had highly limited cardiogenic activity, inadequate to support efficient cardiac repair. Here we reassess the lineage tracing data by investigating the identity of cells immediately after Cre labeling. Our instant lineage tracing approach identifies Kit-expressing cardiomyocytes, which are labeled immediately after tamoxifen induction. In combination with long-term lineage tracing experiments, these data reveal that the large majority of long-term labeled cardiomyocytes are pre-existing Kit-expressing cardiomyocytes rather than cardiomyocytes formed de novo from CSCs. This study presents a new interpretation for the contribution of Kit(+) cells to cardiomyocytes and shows that Kit genetic lineage tracing over-estimates the cardiogenic activity of Kit(+) CSCs.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP671225391-A	Cardiogenol C - CAS 671225-39-1		671225-39-1	Price

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