Home
Resources
Publications
Genomic Cloning and Characterization of a Ricin Gene From Ricinus Communis

Genomic Cloning and Characterization of a Ricin Gene From Ricinus Communis

K C Halling, A C Halling, E E Murray, B F Ladin, L L Houston, R F Weaver

Nucleic Acids Res. 1985 Nov 25;13(22):8019-33.

PMID: 2999712

Abstract:

A genomic clone that specifies a single polypeptide precursor for ricin, a toxic lectin of Ricinus communis (castor bean), was isolated, sequenced and Sl mapped. The gene encodes a 64 kDa precursor which contains, in the following order: a 24 or 35 amino acid signal peptide, the A chain, a 12 amino acid linker peptide, and the B chain. The 5'-end of the ricin mRNA maps approximately 35 bases upstream from the first methionine codon. Two putative TATA boxes and a possible CAAT box lie in the 5'-flanking region. Two possible polyadenylation signals were found in the 3' flanking region. No introns were found, which is typical of other lectin genes that have been sequenced. Southern blot analysis suggests that the castor bean genome contains approximately six ricin-like genes.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP96638287	Ricin A chain from Ricinus communis (castor bean)		96638-28-7	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: