Home
Resources
Publications
α-Glucosidase Inhibition and Antioxidant Activity of an Oenological Commercial Tannin. Extraction, Fractionation and Analysis by HPLC/ESI-MS/MS and (1)H NMR

α-Glucosidase Inhibition and Antioxidant Activity of an Oenological Commercial Tannin. Extraction, Fractionation and Analysis by HPLC/ESI-MS/MS and (1)H NMR

Vera Muccilli, Nunzio Cardullo, Carmela Spatafora, Vincenzo Cunsolo, Corrado Tringali

Food Chem. 2017 Jan 15;215:50-60.

PMID: 27542449

Abstract:

Two batches of the oenological tannin Tan'Activ R, (toasted oak wood - Quercus robur), were extracted with ethanol. A fractionation on XAD-16 afforded four fractions for each extract. Extracts and fractions were evaluated for antioxidant activity (DPPH), polyphenol content (GAE) and yeast α-glucosidase inhibitory activity. Comparable results were obtained for both columns, fractions X1B and X2B showing the highest antioxidant activity. Fractions X1C and X2C notably inhibited α-glucosidase, with IC50=9.89 and 8.05μg/mL, respectively. Fractions were subjected to HPLC/ESI-MS/MS and (1)H NMR analysis. The main phenolic constituents of both X1B and X2B were a monogalloylglucose isomer (1), a HHDP-glucose isomer (2), castalin (3) gallic acid (4), vescalagin (5), and grandinin (or its isomer roburin E, 6). X1C and X2C showed a complex composition, including non-phenolic constituents. Fractionation of X2C gave a subfraction, with enhanced α-glucosidase inhibitory activity (IC50=6.15μg/mL), with castalagin (7) as the main constituent.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP19086750	Castalin		19086-75-0	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: