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Glutathione Production From Mannan-Based Bioresource by mannanase/mannosidase Expressing Saccharomyces Cerevisiae

Glutathione Production From Mannan-Based Bioresource by mannanase/mannosidase Expressing Saccharomyces Cerevisiae

Alex Prima, Kiyotaka Y Hara, Apridah Cameliawati Djohan, Norimasa Kashiwagi, Prihardi Kahar, Jun Ishii, Hideki Nakayama, Fumiyoshi Okazaki, Bambang Prasetya, Akihiko Kondo, Yopi, Chiaki Ogino

Bioresour Technol. 2017 Dec;245(Pt B):1400-1406.

PMID: 28624243

Abstract:

This work aims to produce glutathione directly from mannan-based bioresources using engineered Saccharomyces cerevisiae. Mannan proved to be a valuable carbon source for glutathione production by this organism. Mannan-hydrolyzing S. cerevisiae was developed by heterologous expression of mannanase/mannosidase on its cell surface. This strain efficiently produced glutathione from mannose polysaccharide, β-1,4-mannan. Furthermore, it produced glutathione from locust bean gum (LBG), a highly dense and inexpensive mannan-based bioresource, as sole carbon source. Glutathione productivity from LBG was enhanced by engineering the glutathione metabolism of mannan-hydrolyzing S. cerevisiae. Expression of extracellular mannanase/mannosidase protein combined with intracellular metabolic engineering is potentially applicable to the efficient, environmentally friendly bioproduction of targeted products from mannan-based bioresources.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9036888	Mannan from Saccharomyces cerevisiae		9036-88-8	Price

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