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Gold Nanoparticle as an Electrochemical Label for Inherently Crosstalk-Free Multiplexed Immunoassay on a Disposable Chip

Gold Nanoparticle as an Electrochemical Label for Inherently Crosstalk-Free Multiplexed Immunoassay on a Disposable Chip

Chuan Leng, Guosong Lai, Feng Yan, Huangxian Ju

Anal Chim Acta. 2010 May 7;666(1-2):97-101.

PMID: 20433971

Abstract:

This work proposed a simple, sensitive and low-cost multiplexed immunoassay by combining a disposable chip with gold nanoparticle (AuNP) as an electrochemical label. The immunosensors array as the disposable chip was firstly prepared by immobilizing capture antibodies on different screen printed carbon working electrodes by passive adsorption. With a sandwich mode, the analytes were then bound to the corresponding capture antibodies for further capture of the gold nanoparticle labeled antibodies. Gold nanoparticles were finally electrooxidized in 0.1 M HCl to produce AuCl(4)(-) for differential pulse voltammetric detection. Using human IgG and goat IgG as model targets, under optimal conditions this method achieved linear ranges from 5.0 to 500 and 5.0 to 400 ng mL(-1) with limits of detection of 1.1 and 1.6 ng mL(-1), respectively. This method eliminated completely electrochemical crosstalk between adjacent immunosensors due to the strong adsorption of the AuCl(4)(-) on the printed carbon surfaces. The immunosensors array showed acceptable precision, reproducibility and stability, and could be readily applied to multianalyte determination for clinical diagnostics.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248955	Anti-STUB1/CHIP antibody produced in goat			Price

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