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Gold Nanoparticle-Decorated Reduced-Graphene Oxide Targeting Anti Hepatitis B Virus Core Antigen

Gold Nanoparticle-Decorated Reduced-Graphene Oxide Targeting Anti Hepatitis B Virus Core Antigen

Mohamad Farid Abd Muain, Kooi Hoong Cheo, Muhamad Nadzmi Omar, Amir Syahir Amir Hamzah, Hong Ngee Lim, Abu Bakar Salleh, Wen Siang Tan, Asilah Ahmad Tajudin

Bioelectrochemistry. 2018 Aug;122:199-205.

PMID: 29660648

Abstract:

Hepatitis B virus core antigen (HBcAg) is the major structural protein of hepatitis B virus (HBV). The presence of anti-HBcAg antibody in a blood serum indicates that a person has been exposed to HBV. This study demonstrated that the immobilization of HBcAg onto the gold nanoparticles-decorated reduced graphene oxide (rGO-en-AuNPs) nanocomposite could be used as an antigen-functionalized surface to sense the presence of anti-HBcAg. The modified rGO-en-AuNPs/HBcAg was then allowed to undergo impedimetric detection of anti-HBcAg with anti-estradiol antibody and bovine serum albumin as the interferences. Upon successful detection of anti-HBcAg in spiked buffer samples, impedimetric detection of the antibody was then further carried out in spiked human serum samples. The electrochemical response showed a linear relationship between electron transfer resistance and the concentration of anti-HBcAg ranging from 3.91ngmL-1 to 125.00ngmL-1 with lowest limit of detection (LOD) of 3.80ngmL-1 at 3σm-1. This established method exhibits potential as a fast and convenient way to detect anti-HBcAg.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42414628	HBcAg core human			Price

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