Home
Resources
Publications
Gram-scale Chemical Synthesis of 2'-deoxynucleoside-5'-o-triphosphates

Gram-scale Chemical Synthesis of 2'-deoxynucleoside-5'-o-triphosphates

Anilkumar R Kore, Muthian Shanmugasundaram, Annamalai Senthilvelan, Balasubramanian Srinivasan

Curr Protoc Nucleic Acid Chem. 2012 Jun;Chapter 13:Unit13.10.

PMID: 22700336

Abstract:

A simple, straightforward, reliable, and efficient method for the chemical synthesis of sodium salt of 2'-deoxynucleoside-5'-O-triphosphates (dNTPs), starting from the corresponding nucleoside, is described. This improved "one-pot, three-step" synthetic strategy involves the monophosphorylation of nucleoside, followed by reaction with tributylammonium pyrophosphate and hydrolysis of the resulting cyclic intermediate to provide the corresponding dNTP in good yields (65% to 70%). It is noteworthy that the protocol holds good for both the purine deoxynucleotides, such as 2'-deoxyguanosine-5'-O-triphosphate (dGTP) and 2'-deoxyadenosine-5'-O-triphosphate (dATP), and pyrimidine deoxynucleotides, such as 2'-deoxycytidine-5'-O-triphosphate (dCTP), thymidine-5'-O-triphosphate (TTP), and 2'-deoxyuridine-5'-O-triphosphate (dUTP).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP18423433	Thymidine 5′-triphosphate sodium salt		18423-43-3	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: