Home
Resources
Publications
Graphical Evaluation of Alkylation of Myosin's SH1 and SH2: The N-phenylmaleimide Reaction

Graphical Evaluation of Alkylation of Myosin's SH1 and SH2: The N-phenylmaleimide Reaction

L Xie, W X Li, V A Barnett, M Schoenberg

Biophys J. 1997 Feb;72(2 Pt 1):858-65.

PMID: 9017211

Abstract:

Previous assertions about the effect of alkylation of SH1 and SH2 on the myosin high-salt calcium and EDTA ATPases have been summarized, and a simple procedure for obtaining the fractional labeling of SH1 and SH2 after treatment of myosin with alkylating agents has been derived. A simple graphical procedure for illustrating the degree of preference of a particular alkylating agent for SH1 over SH2 has also been developed. The procedures we developed were validated by applying them to two previously studied compounds, 4-(2-iodoacetamido)-TEMPO and 2,4-dinitrofluorobenzine, and then were used to determine a procedure for maximizing the extent of labeling of SH1 alone by N-phenylmaleimide, a compound not previously studied in this manner. It was found that approximately 80% of the SH1 sites could be alkylated without significant alkylation of SH2.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP25713240	4-(2-Iodoacetamido)-TEMPO		25713-24-0	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: