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GSK-J4-Mediated Transcriptomic Alterations in Differentiating Embryoid Bodies

GSK-J4-Mediated Transcriptomic Alterations in Differentiating Embryoid Bodies

Chanchal Mandal, Sun Hwa Kim, Sung Chul Kang, Jin Choul Chai, Young Seek Lee, Kyoung Hwa Jung, Young Gyu Chai

Mol Cells. 2017 Oct;40(10):737-751.

PMID: 29047260

Abstract:

Histone-modifying enzymes are key players in the field of cellular differentiation. Here, we used GSK-J4 to profile important target genes that are responsible for neural differentiation. Embryoid bodies were treated with retinoic acid (10 μM) to induce neural differentiation in the presence or absence of GSK-J4. To profile GSKJ4-target genes, we performed RNA sequencing for both normal and demethylase-inhibited cells. A total of 47 and 58 genes were up- and down-regulated, respectively, after GSK-J4 exposure at a log2-fold-change cut-off value of 1.2 (p-value < 0.05). Functional annotations of all of the differentially expressed genes revealed that a significant number of genes were associated with the suppression of cellular proliferation, cell cycle progression and induction of cell death. We also identified an enrichment of potent motifs in selected genes that were differentially expressed. Additionally, we listed upstream transcriptional regulators of all of the differentially expressed genes. Our data indicate that GSK-J4 affects cellular biology by inhibiting cellular proliferation through cell cycle suppression and induction of cell death. These findings will expand the current understanding of the biology of histone-modifying enzymes, thereby promoting further investigations to elucidate the underlying mechanisms.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1373423530	GSK-J4		1373423-53-0	Price

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