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Heat Shock Protein 70 Modulates Neural Progenitor Cells Dynamics in Human Neuroblastoma SH-SY5Y Cells Exposed to High Glucose Content

Heat Shock Protein 70 Modulates Neural Progenitor Cells Dynamics in Human Neuroblastoma SH-SY5Y Cells Exposed to High Glucose Content

Leila Salimi, Reza Rahbarghazi, Vahab Jafarian, Çıgır Biray Avci, Bakiye Goker Bagca, Neslihan Pinar Ozates, Majid Khaksar, Alireza Nourazarian

J Cell Biochem. 2018 Aug;119(8):6482-6491.

PMID: 29345335

Abstract:

In the current experiment, detrimental effects of high glucose condition were investigated on human neuroblastoma cells. Human neuroblastoma cell line SH-SY5Y were exposed to 5, 40, and 70 mM glucose over a period of 72 h. Survival rate and the proliferation of cells were analyzed by MTT and BrdU incorporation assays. Apoptosis was studied by the assays of flow cytometry and PCR array. In order to investigate the trans-differentiation capacity of the cell into mature neurons, we used immunofluorescence imaging to follow NeuN protein level. The transcription level of HSP70 was shown by real-time PCR analysis. MMP-2 and -9 activities were shown by gelatin Zymography. According to data from MTT and BrdU incorporation assay, 70 mM glucose reduced cell viability and proliferation rate as compared to control (5 mM glucose) and cells treated with 40 mM glucose (P < 0.05). Cell exposure to 70 mM glucose had potential to induced apoptosis after 72 h (P < 0.05). Our results also demonstrated the sensitivity of SH-SY5Y cells to detrimental effects of high glucose condition during trans-differentiation into mature neuron-like cells. Real-time PCR analysis confirmed the expression of HSP70 in cells under high content glucose levels, demonstrating the possible cell compensatory response to an insulting condition (pcontrol vs 70 mM group <0.05). Both MMP-2 and -9 activities were reduced in cells being exposed to 70 mM glucose. High glucose condition could abrogate the dynamics of neural progenitor cells. The intracellular level of HSP70 was proportional to cell damage in high glucose condition.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4241273	MMP Control-5			Price

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