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Hemin/G-quadruplex-based DNAzyme Concatamers for in Situ Amplified Impedimetric Sensing of copper(II) Ion Coupling With DNAzyme-catalyzed Precipitation Strategy

Hemin/G-quadruplex-based DNAzyme Concatamers for in Situ Amplified Impedimetric Sensing of copper(II) Ion Coupling With DNAzyme-catalyzed Precipitation Strategy

Mingdi Xu, Zhuangqiang Gao, Qiaohua Wei, Guonan Chen, Dianping Tang

Biosens Bioelectron. 2015 Dec 15;74:1-7.

PMID: 26093122

Abstract:

A new signal-amplification strategy based on copper(II) (Cu(2+))-dependent DNAzyme was developed for sensitive impedimetric biosensing of Cu(2+) in aqueous solution by coupling with target-induced formation of hemin/G-quadruplex-based DNAzyme and enzymatic catalytic precipitation technique. Initially, the target analyte cleaved the Cu(2+)-specific DNAzyme to generate an initiator strand on the sensor. Thereafter, the initiator strand underwent an unbiased strand-displacement reaction between hairpin probes in turn to construct a nicked double-helix, accompanying the formation of hemin/G-quadruplex DNAzyme on the long duplex DNA. The newly formed DNAzyme could oxidize the 4-chloro-1-naphthol (4-CN) to produce an insoluble precipitation on the sensor, thus resulting in a local alteration of the conductivity. Under the optimal conditions, the resistance increased with the increasing Cu(2+) in the sample, and exhibited a wide dynamic working range from 0.1 pM to 5.0 nM with a detection limit of 60 fM. The methodology also displayed a high selectivity for Cu(2+) relative to other potentially interfering ions owing to the highly specific Cu(2+)-dependent DNAzyme, and was applicable for monitoring Cu(2+) in real river samples. Thus, our strategy has a good potential in the environment surveys.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411223	4-Chloro-1-naphthol solution			Price

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