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Heterogeneity of Protein Labeling With a Fluorogenic Reagent, 3-(2-furoyl)quinoline-2-carboxaldehyde

Heterogeneity of Protein Labeling With a Fluorogenic Reagent, 3-(2-furoyl)quinoline-2-carboxaldehyde

Alexander V Stoyanov, Hossein Ahmadzadeh, Sergey N Krylov

J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Nov 25;780(2):283-7.

PMID: 12401353

Abstract:

Fluorogenic reagents are used for protein labeling when high-sensitivity fluorescence detection is required. Similar to traditional labeling with activated fluorescent dyes, such as fluorescein isothiocyanate, a fluorogenic reaction is expected to change the physical-chemical properties of proteins. Knowledge of these changes may be essential for efficient separation and identification of labeled proteins. Here we studied the effect of labeling of myoglobin with a fluorogenic reagent on the acid-base properties of the protein. The fluorogenic reagent used was 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ). In slab-gel isoelectric focusing, we found that the labeling reaction generated at least six species with pI values lower than that of non-labeled myoglobin. These species can be identified as products of progressive labeling of myoglobin with one to six FQ molecules. The same series of FQ-labeled species were observed when the reaction products were analyzed by capillary zone electrophoresis. The comparison of experimental and theoretical pI values allowed us to elucidate the labeling pattern--the number of FQ molecules corresponding to each labeled product detected by isoelectric focusing.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP126769015	3-(2-Furoyl)quinoline-2-carboxaldehyde		126769-01-5	Price

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