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Hexadecylphosphocholine and 2-modified 1,3-diacylglycerols as Effectors of Phospholipase D

Hexadecylphosphocholine and 2-modified 1,3-diacylglycerols as Effectors of Phospholipase D

N Dittrich, R Haftendorn, R Ulbrich-Hofmann

Biochim Biophys Acta. 1998 Mar 30;1391(2):265-72.

PMID: 9555051

Abstract:

The kinetic behaviour of phospholipase D (PLD) from cabbage has been studied in the presence of several substrate-like compounds such as hexadecylphosphocholine (HPC) and 1,3-didodecanoylglycero-2-phosphatides. 1,3-Didodecanoyl- glycero-2-phosphocholine (1,3-DiC12PC) was found being not cleft by PLD, whereas HPC is hydrolyzed by PLD with small rate. The plot of initial velocity vs. substrate concentration for HPC is more sigmoidal than those for the common substrate phosphatidylcholine (PC)/sodium dodecylsulfate (SDS) (1:0.5) or the short-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DiC6PC). The anionic amphiphiles 1,3-didodecanoylglycero-2-sulfate and 1,3-didodecanoylglycero-2-phosphate act as activators of PLD towards PC similar to SDS. In contrast, 1,3-DiC12PC shows inhibitory properties with an increase in the sigmoidicity of the initial velocity as a function of substrate concentration in the PC/SDS assay. Also HPC inhibits the hydrolysis of PC/SDS, whereas it acts as activator or inhibitor in the hydrolysis of DiC6PC. The results suggest that PLD possesses two substrate-binding sites, where one binds substrate in function of an effector without catalytic activity while the other is the catalytic site. HPC and 1,3-DiC12PC are assumed to compete with the substrate for both binding sites with effects depending on the ratio of concentrations and affinities of substrates and effectors.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP34506677	1,2-Dihexanoyl-sn-glycero-3-phosphocholine		34506-67-7	Price

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