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High-level Expression and Characterization of Carboxypeptidase Y From Saccharomyces Cerevisiae in Pichia Pastoris GS115

High-level Expression and Characterization of Carboxypeptidase Y From Saccharomyces Cerevisiae in Pichia Pastoris GS115

Xianhong Yu, Chao Zhai, Xing Zhong, Wei Tang, Xiaojuan Wang, Hu Yang, Wanping Chen, Lixin Ma

Biotechnol Lett. 2015 Jan;37(1):161-7.

PMID: 25214228

Abstract:

Carboxypeptidase Y is widely used in peptide sequencing and mass spectrometry. PRC1 coding for proteinase C from Saccharomyces cerevisiae was expressed in Pichia pastoris GS115 as procarboxypeptidase Y with a yield of ~605 mg/l in shake-flasks after 168 h induction with 1 % (v/v) methanol. This precursor of carboxypeptidase Y was cleaved by endogenous proteinases of P. pastoris and released into the fermentation broth as active carboxypeptidase Y within 2 weeks at 10 °C, which facilitated the preparation of mature carboxypeptidase Y. The recombinant enzyme was purified. It was optimally active at 30 °C and pH 6.0, with an optimal activity of ~305 U/mg using benzyloxycarbonyl-L-phenylalanyl-L-leucine as substrate. This is the first report about high-level expression and activation of carboxypeptidase Y in P. pastoris.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9046677	Carboxypeptidase Y from baker's yeast (S. cerevisiae)		9046-67-7	Price

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