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High Level Expression and Crystallization of Recombinant Human Cathepsin S

D Brömme, M E McGrath

Protein Sci. 1996 Apr;5(4):789-91.

PMID: 8845771

Abstract:

We have expressed active human cathepsin S to 60 mg/L in Sf9 cells using a baculovirus system. Production of milligram quantities has facilitated crystallographic studies to determine the structure of this enzyme, which has unique properties among lysosomal cysteine proteinases. Recombinant, irreversibly inhibited cathepsin S was crystallized from ammonium phosphate at 17 degrees C. The crystals diffract to at least 2.3 A, and belong to the orthorhombic crystal system with a primitive lattice. Approximate cell dimensions are: a = 37.7 A, b = 73.9 A, and c = 106.7 A. There is most likely one molecule per asymmetric unit.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42412611 Cathepsin S Active human Cathepsin S Active human Price
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