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High-level Expression of Codon-Optimized Thielavia Terrestris Cutinase Suitable for Ester Biosynthesis and Biodegradation

High-level Expression of Codon-Optimized Thielavia Terrestris Cutinase Suitable for Ester Biosynthesis and Biodegradation

Xiaojie Duan, Zhengqiang Jiang, Yu Liu, Qiaojuan Yan, Man Xiang, Shaoqing Yang

Int J Biol Macromol. 2019 Aug 15;135:768-775.

PMID: 31129216

Abstract:

A codon-optimized cutinase gene (TtCutopt) from Thielavia terrestris was over-expressed in Pichia pastoris. An extracellular activity reached 10,200 U/mL using high cell density fermentation. The optimal pH and temperature of TtCutopt were 7.0 and 50 °C, respectively. It displayed high stability over a wide range of pH from 3.0 to 11.0 and up to 85 °C. Among tested p-nitrophenyl esters and triglycerides, TtCutopt showed the highest activity towards p-nitrophenyl butyrate and tributyrin, with specificity activity of 2322.4 U/mg and 1152.5 U/mg, respectively. It was extremely stable in organic solvents and surfactants. TtCutopt efficiently catalyzed the synthesis of butyl butyrate, hexyl butyrate, butyl hexanoate and hexyl hexanoate with esterification efficiency of >95%. Furthermore, it catalyzed the degradation of >90% of dimethyl phthalate, diethyl phthalate, dipropyl phthalate and dibutyl phthalate to release their corresponding monoalkyl phthalates within 24 h. Thus, high yield, high stability, and esterification efficiency of TtCutopt make it an attractive candidate for ester biosynthesis and biodegradation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP626824-A	Butyl hexanoate		626-82-4	Price
AP6378650	Hexyl hexanoate		6378-65-0	Price

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